比菲多细菌的利波泰可酸和虚假的ELISA反应性在阿斯伯吉路斯抗原检测中的反应性
Monique A S H Mennink-Kersten1, Rocus R Klont, Adilia Warris
1Department of Medical Microbiology, University Medical Centre Nijmegen, Netherland.
Lancet (London, England)
|January 31, 2004
概括
婴儿侵入性阿斯伯吉洛症的错误阳性ELISA测试可能是由Bifidobacterium lipoteichoic acid引起的. 本研究探讨了这种联系,以提高诊断准确度,防止不必要的治疗.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 菌类学 菌类学是指菌类学.
- 微生物学 微生物学
背景情况:
- 侵入性阿斯伯吉洛症诊断依赖于检测银河,但会发生错误阳性.
- 新生儿和婴儿特别容易受到假阳性ELISA结果的影响.
- 阿斯伯吉illus galactomannan 与细菌成分具有分子相似之处.
研究的目的:
- 调查Bifidobacterium lipoteichoic acid导致侵袭性阿斯伯吉洛症错误阳性ELISA结果的假设.
- 探索一种方法来区分婴儿的真假阳性ELISA结果.
- 减少儿科患者不必要的预防性治疗.
主要方法:
- 假设阿斯伯吉勒斯银河曼南和Bifidobacterium lipoteichoic 酸之间的分子模仿.
- 考虑到Bifidobacterium在新生儿肠道中的高度殖民.
- 检查细菌成分在不成熟的肠道粘膜中可能发生的转移.
主要成果:
- 该研究假设Bifidobacterium lipoteichoic acid可能作为ELISA单克隆抗体的表位.
- 新生儿的不成熟的肠粘膜可能允许细菌成分的转移.
- 这种转移可能导致错误的阳性ELISA反应.
结论:
- 双杆菌脂铁醇酸是婴儿中错误阳性银河ELISA结果的潜在原因.
- 识别这种交叉活性可能会提高诊断特异性.
- 这可能会防止在脆弱的儿科群体中过度治疗侵入性阿斯伯吉洛症.
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