鼠类细胞巨乳病毒感染增加了前动脉样硬化基因的大动脉表达
Mary Susan Burnett1, Sarfraz Durrani, Eugenio Stabile
1Cardiovascular Research Institute, MedStar Research Institute, Washington Hospital Center, Washington, DC 20010, USA. mary.s.burnett-miller@medstar.net
Circulation
|February 6, 2004
概括
鼠类细胞巨乳病毒 (MCMV) 感染增加了小鼠的动脉样损伤大小和亲动脉体基因表达. 这些发现表明细胞巨核病毒在心血管疾病的发展和进展中起着作用.
科学领域:
- 心血管疾病研究研究
- 传染病免疫学 传染病免疫学
- 动脉样硬化病原体的产生
背景情况:
- 感染在心血管疾病中的作用仍在争论中.
- 之前的研究表明,小鼠细胞巨乳病毒 (MCMV) 感染在apolipoprotein E缺陷 (apoE-/-) 的小鼠中加剧动脉样硬化.
- 这项研究调查了MCMV对大动脉基因表达和病变发展的影响.
研究的目的:
- 为了确定MCMV感染是否诱导大动脉基因表达的亲动脉原性变化.
- 为了检查MCMV感染对胆固醇养小鼠的大动脉损伤区域的影响.
主要方法:
- 小鼠 (apoE-/和C57BL/6J) 被感染了MCMV.
- 使用RNA提取,Affymetrix芯片和TaqMan RT-PCR分析了大动脉基因表达.
- 在胆固醇养小鼠中,通过油红色O染色评估了大动脉鼻损伤区域.
主要成果:
- 在年轻小鼠中,MCMV感染改变了关键的动脉动脉基因 (MCP-1,MIG,IP-10) 的表达.
- 在老年apoE-/-小鼠中,感染增加了MCP-1表达,这些小鼠具有现有的病变.
- 在MCMV感染后的胆固醇养小鼠中,动脉样损伤面积显著增加.
结论:
- 经证实,MCMV感染增加了动脉样硬化病变区域和动脉样基因的表达.
- 这些发现阐明了细胞巨核病毒对动脉样硬化开始和进展的潜在机制.
- 这些结果支持了感染在动脉样硬化的疾病中起着重要作用的假设.
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