相关实验视频
Updated: Jul 16, 2026
07:59
Folding and Characterization of a Bio-responsive Robot from DNA Origami
Published on: December 3, 2015
一个1.7千基单链DNA,折叠成一个纳米级的八面体
William M Shih1, Joel D Quispe, Gerald F Joyce
1Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. wmshih@scripps.edu
Nature
|February 13, 2004
概括
研究人员设计了一种新的DNA原形技术,以创建自组装的DNA八面体. 这种方法允许复杂的DNA纳米结构的可扩展生产,使每个组件具有独特的可定位性.
科学领域:
- 纳米技术纳米技术
- 分子生物学分子生物学
- 生物物理学的生物物理.
背景情况:
- 分子自我组装利用特定的DNA基配对来构建纳米结构.
- 现有的3D DNA对象缺乏适合基于聚合酶的克隆的拓,限制了大规模生产.
- 可扩展的生产对于推进DNA纳米技术应用至关重要.
研究的目的:
- 设计和合成一种可接受聚合酶放大的DNA分子,用于创建3D几何DNA对象.
- 开发一种将这种DNA分子折叠成稳定,复杂的纳米结构的方法.
- 为了使DNA纳米结构中的单个组件具有独特的可定位性.
主要方法:
- 一个1,669核酸单链DNA分子的设计和合成.
- 将DNA分子折叠成八面体结构,使用五种合成的寡氧核酸通过变性-重制.
- 低温电子显微镜用于折叠DNA八面体的结构特征.
主要成果:
- 成功地将DNA分子折叠成直径约22纳米的空心八面体.
- 八面体结构由12个支架组成,在6个四向连接处连接.
- 每个支架都有一个独特的基对序列,允许个别的可定位性.
结论:
- 一种新的,可放大的DNA原始创作方法已经开发出来,用于构建复杂的3DDNA纳米结构.
- 合成的DNA八面体通过冷电子显微镜进行结构验证.
- 单个支架的独特序列可定位性为先进的分子编程和纳米尺度设备制造开辟了可能性.
相关概念视频
01:16The DNA Helix
Overview
01:16The DNA Helix
Overview
02:21Chromatin Packaging
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter?
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
01:07The DNA Helix
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
01:19The Nucleosome
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
01:32Chromatin Packaging
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...