相关实验视频
Updated: Jul 10, 2026

10:19
Derivation of Mouse Trophoblast Stem Cells from Blastocysts
Published on: June 8, 2010
撤回:证据表明,一个多能的人类胚胎干细胞系来自克隆的胚胎细胞
Woo Suk Hwang1, Young June Ryu, Jong Hyuk Park
1College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. hwangws@snu.ac.kr
概括
研究人员通过体细胞核转移 (SCNT) 从克隆胚胎细胞中获得了人类胚胎干细胞 (ES) 细胞系. 这些SCNT-人类ES细胞具有多能性,与捐赠者基因相同,支持SCNT作为人类ES细胞生成的可行方法.
科学领域:
- 生殖生物学 生殖生物学
- 干细胞科学 干细胞科学
- 遗传学 是一个遗传学.
背景情况:
- 实体细胞核转移 (SCNT) 能够创造出基因相同的动物.
- 获得人类胚胎干细胞对于再生医学和疾病建模至关重要.
研究的目的:
- 使用SCNT从克隆的人类母细胞中提取和特征化一个多能的人类胚胎干细胞系.
- 确认SCNT起源并评估衍生细胞的多能性和遗传稳定性.
主要方法:
- 在人体卵细胞上进行了体细胞核转移 (SCNT).
- 衍生芽细胞被用来建立胚胎干细胞系.
- 鉴定包括形态学,细胞表面标记物,体外分化 (胚胎体),体内瘤形成,型和印记分析.
主要成果:
- 一个多能的人类ES细胞系 (SCNT-hES-1) 从克隆的胚胎细胞成功衍生出来.
- SCNT-hES-1细胞表现出典型的ES细胞特征,包括多能性和分化能力到所有三个胚胎层.
- 细胞在70个通道中保持了正常的细胞类型和捐赠细胞的遗传特征.
- 印记分析支持SCNT起源,尽管不能完全排除 partenogenetic起源.
结论:
- SCNT是一种可行的方法,用于产生多能人体ES细胞.
- 衍生的SCNT-hES-1细胞系在遗传上稳定,并保持多能性.
- 这些发现对治疗克隆和干细胞研究有影响.
相关概念视频
Reproductive Cloning
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Fertilization
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
Cleavage and Blastulation
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
Reproductive Cloning
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Zygotic Development And Stem Cell Formation
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
Maintenance of the ES Cell State
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

