栗子炎真菌的低毒性是由传染性病毒cDNA赋予的
1Department of Molecular Oncology and Virology, Roche Institute of Molecular Biology, Nutley, NJ 07110.
概括
某些病毒减少了栗子菌Cryphonectria parasitica的破坏性影响. 这些低毒性菌株可以被改造,提供一种新的生物控制策略来对抗这种植物病原体.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物病理学 植物病理学
- 病毒学 病毒学
背景情况:
- 栗子病是由Cryphonectria parasitica引起的,破坏了栗子种群.
- 常常感染病毒双链RNA (dsRNA) 的C.parasitica低病毒性菌株的病原性降低.
- 这些低毒性菌株可以通过解剖形成将毒性菌株转化为不那么有害的状态.
研究的目的:
- 确认病毒dSRNA是C. parasitica.中低病毒发病的致病原体.
- 为了证明为生物控制而设计低毒性真菌菌株的可行性.
主要方法:
- 病毒性C.寄生虫的转化与低病毒性相关的病毒RNA的cDNA副本.
- 从集成的cDNA中复活细胞质dRNA.
- 测试复活的dsRNA转化毒性菌株的能力.
主要成果:
- 转变的菌株表现出完整的低毒性表型.
- 复活的dsRNA成功地将兼容的毒性菌株转化为低毒性.
- 病毒dSRNA被证实是低病毒性的致病因子.
结论:
- 病毒dSRNA绝对是Cryphonectria parasitica中低毒性的原因.
- 工程低毒性真菌菌株是一种可行的生物控制方法,用于栗病.
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