Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Clock gene signature predicts insomnia and links to sleep/circadian parameters.

Translational psychiatry·2026
Same author

Quantifying sleep wake rhythms in the hospital environment with digital technologies.

NPJ digital medicine·2026
Same author

Glucose is dynamically regulated by time of day in humans and Drosophila.

PLoS biology·2026
Same author

Endocytosis at the mouse blood brain barrier is elevated during sleep.

bioRxiv : the preprint server for biology·2026
Same author

Sleep-dependent clearance of brain lipids by peripheral blood cells.

Nature·2026
Same author

RNAi-based screen for pigmentation in <i>Drosophila melanogaster</i> reveals regulators of brain dopamine and sleep.

iScience·2026

相关实验视频

Updated: Jun 10, 2026

Proboscis Extension Response (PER) Assay in Drosophila
23:42

Proboscis Extension Response (PER) Assay in Drosophila

Published on: April 29, 2007

用蛋白质酸酶2A对Drosophila PERIOD蛋白进行后翻译调节.

Sriram Sathyanarayanan1, Xiangzhong Zheng, Rui Xiao

  • 1Howard Hughes Medical Institute, Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.

Cell
|February 26, 2004
PubMed
概括

蛋白酸酶2A (PP2A) 调节昼夜时钟蛋白的PER水平. 节奏性PP2A表达对于PER循环和保持果强大的昼夜节律至关重要.

科学领域:

  • 时间生物学 时间生物学
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 控制昼夜钟蛋白水平波动的精确的转录后机制在很大程度上是未知的.
  • 了解这些机制对于破译昼夜节律调节至关重要.

研究的目的:

  • 研究蛋白酸酶2A (PP2A) 在周期蛋白 (PER) 的循环表达中的作用.
  • 为了阐明PP2A对昼夜时钟功能的贡献.

主要方法:

  • 使用S2细胞和成年果头进行实验分析.
  • 研究了PP2A调控子单元 (TWS,WDB) 和催化子单元对PER蛋白的影响.
  • 评估了PER酸化在体外和体内.
  • 检查了转基因果的时钟神经元中的行为节律和PER表达 (tws突变,过度表达线).

主要成果:

  • PP2A调控子单位TWS和WDB在S2单元中准并稳定PER.
  • 在成年头中,TWS表达周期是节奏的.
  • 在tws中的突变导致PER积累的延迟;tws过度表达缩短昼夜节律.
  • 降低PP2A活性会导致PER表达的减少,长时间,以及时钟神经元中的心律失常.

更多相关视频

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
07:23

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster

Published on: January 26, 2024

Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection
07:15

Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection

Published on: January 19, 2024

相关实验视频

Last Updated: Jun 10, 2026

Proboscis Extension Response (PER) Assay in Drosophila
23:42

Proboscis Extension Response (PER) Assay in Drosophila

Published on: April 29, 2007

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
07:23

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster

Published on: January 26, 2024

Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection
07:15

Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection

Published on: January 19, 2024

  • 过度表达PP2A催化子单元会破坏行为节奏,并导致构成性核PER局部化.
  • PP2A在体外和体内都会影响PER酸化.

    • 结论:

    • PP2A在PER蛋白循环的转录后调节中起着至关重要的作用.
    • PP2A的节奏表达对于驱动PER蛋白的循环和保持强大的昼夜节律至关重要.