在Streptomyces resistomycificus中编码resistomycin生物合成的基因集群;探索超越线性和循环模式的多基基因循环
Kathrin Jakobi1, Christian Hertweck
1Hans-Knöll-Institute for Natural Products Research, Department of Bioorganic Synthesis, Beutenbergstrasse 11a, 07745 Jena, Germany.
Journal of the American Chemical Society
|February 26, 2004
概括
研究人员从Streptomyces resistomycificus中识别了抗菌素基因集群,揭示了这种药理学上相关的光盘多基基的独特生物合成途径. 这一发现提供了对新型多基化物循环机制的见解.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 自然产品化学 自然产品化学
背景情况:
- 抗菌素是一种来自Streptomyces resistomycificus的独特的光盘状多基基,具有药理学意义.
- 细菌芳香多基基类通常是线性或角形的,与抗菌素的独特结构不同.
研究的目的:
- 为了确定负责抗菌素生物合成的基因集群.
- 为了阐明resistomycin独特的光盘状结构和循环的分子基础.
主要方法:
- 来自Streptomyces resistomycificus的一个宇宙图书馆的异质表达.
- 查代谢物光,以确定阳性克隆.
- 对已识别的基因集群 (rem) 的生物信息分析.
主要成果:
- 确定了完整的抵抗菌素 (REM) 基因集群.
- 集群中含有不寻常的II型多基酸合成酶 (PKS) 组件,包括一个假定的malonyl-CoA:ACP转化酶 (MCAT) 与模块化PKS AT域相同.
- 获得了对产生 discoid polyketide 结构的循环化机制的新见解.
结论:
- 已识别的雷姆基因集群为了解抵抗菌素生物合成提供了一个平台.
- PKS机械的独特特点为多基基的多样性和工程提供了新的视角.
- 这项研究揭示了形成罕见的光盘状多基基结构的分子基础.
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