Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jul 9, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

具有调节电荷密度和二硫化物交叉链接的区块性阴性聚复合物,旨在增强基因表达.

Kanjiro Miyata1, Yoshinori Kakizawa, Nobuhiro Nishiyama

  • 1Department of Materials Science and Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.

Journal of the American Chemical Society
|February 26, 2004
PubMed
概括
此摘要是机器生成的。

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Fabrication of TiO<sub>2</sub> Nanoparticles with High Colloidal Stability through Surface Modification via Biocompatible Block Copolymers and Heating.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Domino Polymerization for the Synthesis of Reductively Degradable Poly(disulfide)s With Arbitrary Side-Chain Structures.

Angewandte Chemie (International ed. in English)·2026
Same author

Dietary medium chain triglycerides impairs orexigenic action of ghrelin in mice.

Frontiers in endocrinology·2026
Same author

Iatrogenic Right Ventricular Perforation Following an Endomyocardial Biopsy for Fulminant Myocarditis.

Cureus·2026
Same author

Interfacial photocycloaddition polymerization: a synthetic approach for structurally functionalized degradable polymer particles from naturally derived monomers.

Chemical science·2026
Same author

Construction of Robust Cross-Linked Polymer Shells in Interfacial Photocycloaddition Polymerization: Trifunctional vs Tetrafunctional Monomers.

Langmuir : the ACS journal of surfaces and colloids·2025

研究人员开发了一种稳定的区块 catiomer 复合体,用于高效的 DNA 输送. 控制电荷和二硫化物交联改善了稳定性和细胞内DNA释放,显著提高了转染效率.

科学领域:

  • 生物材料科学 生物材料科学
  • 基因传递系统是基因传递系统.
  • 聚合物化学 聚合物化学

背景情况:

  • 为基因传递开发有效的非病毒载体仍然是一个挑战.
  • 控制多重复性稳定性和细胞内DNA释放对于转染效率至关重要.

研究的目的:

  • 设计具有可调节电荷和交叉连接密度的二硫化物交叉连接块阴性聚合物.
  • 调查化方法对多重复合体稳定性,DNA释放和转染效率的影响.

主要方法:

  • 使用SPDP或Traut的试剂,对聚乙烯甘醇) -聚乙烯氨酸 (PEG-PLL) 进行硫化.
  • 与等离子体DNA (pDNA) 复合,形成二硫化物交联多重复合体 (~100 nm).
  • 在氧化条件下对多复合体稳定性和在还原条件下对DNA释放的评估.

主要成果:

  • 这两种硫化方法都在细胞外氧化条件下产生了稳定的多复合体.
  • 在细胞内还原条件下,SPDP-thiolated多复合体表现出高效的pDNA释放.
  • 与特劳特的试剂类型相比,SPDP-thiolated多复合体在293T细胞中显示出大约50倍的转染效率.

结论:

更多相关视频

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
06:21

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Published on: May 7, 2018

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids
07:14

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids

Published on: May 16, 2025

相关实验视频

Last Updated: Jul 9, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
06:21

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Published on: May 7, 2018

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids
07:14

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids

Published on: May 16, 2025

  • 阴离子电荷和二硫化物交联密度之间的平衡对于有效的pDNA传递和释放至关重要.
  • 通过SPDP介导的化为开发高性能基因传递载体提供了一个有前途的策略.
  • 优化的多重体设计增强了细胞内DNA的传递,从而显著提高了传染率.