在八个真核生物基因组中,瓜诺辛的分布和氧化抵抗
Keith A Friedman1, Adam Heller
1Department of Chemical Engineering and the Texas Materials Institute, University of Texas at Austin, Austin, Texas 78712-0231, USA. kafriedman@mail.utexas.edu
Journal of the American Chemical Society
|February 26, 2004
概括
含有较低瓜诺辛 (G) 含量的基因组对氧化损伤具有更强的抵抗力. 在许多物种中,必需的外显子受到G丰富的非编码区域的保护,从而提高了基因组的稳定性.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细胞不断产生反应性氧物种,这些物种会损害DNA.
- 瓜诺辛 (G) 分数及其分布影响基因组抗氧化稳定性.
- 较低的G含量可能会增加氧化抵抗,称为"高贵化".
研究的目的:
- 为了调查基因组是否"高贵化"抵御氧化.
- 为了确定非必不可少的基因组区域是否充当氧化物清理器.
- 为了估计不同物种中外子,内子和基因间域的相对瓜诺辛氧化率.
主要方法:
- 瓜诺辛 (G) 含量和分布的比较基因组分析.
- 在外子,内子和基因间域中估计相对瓜诺辛氧化速率.
- 将氧化速率与具有均G分布的假设标准基因组进行比较.
主要成果:
- 在Caenorhabditis,Arabidopsis,Saccharomyces,Schizosaccharomyces和Plasmodium中,前子是"高贵化" (更耐氧化) 的.
- 在这些物种和Drosophila中,内部和跨基因域是"高贵的".
- 外基因氧化率低于贵族化物种的标准基因组; 杆菌显示了标准速率的一半.
- 人类和飞的外基因并没有被化,而是受到G丰富的内基因和基因间域的保护.
- 与标准相比,脑动物基因组G分布没有保护性.
结论:
- 基因组表现出不同程度的"高贵化"和保护策略,防止氧化DNA损伤.
- 非必不可少的基因组区域,特别是内子和跨基因域,可以充当氧化清除剂,保护必不可少的外基因.
- 瓜诺辛的分布,而不仅仅是其整体分数,对于基因组氧化抵抗至关重要.
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