人类脂肪系 stromal 细胞分泌的血管造生和抗apoptotic 因素
Jalees Rehman1, Dmitry Traktuev, Jingling Li
1Krannert Institute of Cardiology, Indiana Center for Vascular Biology & Medicine, Indianapolis, Ind 46202, USA.
Circulation
|March 3, 2004
概括
皮下脂肪 stromal 细胞 (ASC) 是心血管疾病治疗的一个有前途的来源. 这些细胞增强血管生长和生存,为促进血管生成提供了一种新的治疗选择.
科学领域:
- 再生医学是一种再生医学.
- 心血管研究研究心血管研究
- 细胞疗法细胞疗法
背景情况:
- 心血管疾病治疗正在探索自主细胞输送以促进血管生成.
- 细胞数量有限可能会阻碍当前基于细胞的疗法的有效性.
- 来自皮下脂肪的脂肪系 stromal 细胞 (ASC) 提供了一个新的,可访问的细胞来源.
研究的目的:
- 研究从人体皮下脂肪中获得的非脂肪细胞 stromal 细胞作为治疗性血管生成的来源的潜力.
- 描述ASCs的生长因子分泌特征.
- 评估ASCs在体外和体内血管生成和抗亡作用.
主要方法:
- 人体皮下脂肪 stromal 细胞 (ASC) 的隔离和流动细胞计特征.
- 在正常和缺氧条件下,ASC对生长因子分泌量 (VEGF,HGF,TGF-β) 的量化.
- 评估条件介质对内皮细胞增殖和亡的影响.
- 在体内评估ASCs在缺血后肢的小鼠模型.
主要成果:
- 这些ASC分泌出大量的VEGF,HGF和TGF-β.
- 缺氧和VEGF基因传染明显增加了ASCs的VEGF分泌.
- 来自缺氧ASC的条件介质促进了内皮细胞的生长,并减少了亡.
- 人类ASC治疗显著改善了患有缺血后肢病的小鼠的输液.
结论:
- 皮下ASCs具有血管新生和抗亡的潜力,这是由于协同作用的益血管新生生长因子的分泌.
- 由缺氧调节的本源或转导ASCs代表了增强血管生成的新治疗策略.
- 这些发现支持使用自主ASC用于心血管保护和再生.
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