失活hCDC4可以导致染色体不稳定
Harith Rajagopalan1, Prasad V Jallepalli, Carlo Rago
1The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.
Nature
|March 6, 2004
概括
hCDC4基因的突变导致染色体不稳定,这是人类癌症的标志. 这种与染色体传播缺陷相关的不稳定性,可以在恶性转化之前发生.
科学领域:
- 遗传学 遗传学 是一个
- 癌症生物学 癌症生物学
- 细胞生物学 细胞生物学
背景情况:
- 状体,一个异常的染色体数量,是人类癌症的一个公认的标志.
- 驱动动体积的潜在机制在很大程度上是未知的.
研究的目的:
- 为了确定对人类癌症中无体积症负责的遗传变化.
- 研究hCDC4在维持染色体稳定中的作用.
主要方法:
- 在结直肠癌样本中识别hCDC4基因突变.
- 在结直肠癌细胞中对hCDC4的向基因破坏.
- 对染色体不稳定性,微核形成和细胞周期进展的分析.
主要成果:
- 在人类结直肠癌和前体病变中发现了hCDC4的突变.
- hCDC4的遗传失活导致了微核和染色体的不稳定.
- 观察到的表型与基因相执行和染色体传播的缺陷有关,这些缺陷依赖于环林E.
结论:
- 特定的基因变异,如hCDC4中的变异,可以在很大比例的人类癌症中引起染色体不稳定.
- 染色体不稳定可能发生在瘤发生的早期,在恶性转变之前.
- hCDC4通过调节环林E.在保持染色体稳定方面发挥着关键作用.
相关概念视频
01:11Genetic Lingo
Overview
02:36DNA Damage can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
02:41Lethal Alleles
Agouti: A Lethal Allele
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
02:36DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
01:25Hepatitis
Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...