相关实验视频
Updated: May 8, 2026

14:43
Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
Published on: July 23, 2014
滚动叶1的微RNA介导的压缩指定了玉米叶的极性
Michelle T Juarez1, Jonathan S Kui, Julie Thomas
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Nature
|March 6, 2004
概括
微RNAs (miRNAs) 作为发育信号起作用. 微RNA166是一种保存的极化信号,通过调节植物中的HD-ZIPIII基因表达来定义叶子极性.
科学领域:
- 植物发育生物学植物发育生物学
- 分子遗传学 分子遗传学
- 微RNA的功能是微RNA的功能.
背景情况:
- 植物叶子的发育涉及轴的规范.
- 微RNAs (miRNAs) 是参与发育的调节分子.
- 第三类家庭主体/氨酸拉链 (HD-ZIPIII) 基因指定了轴叶的命运.
研究的目的:
- 为了研究miRNA166在植物叶子极性中的作用.
- 为了确定miRNA166是否起到极化信号的作用.
- 了解miRNA166在叶子发育过程中的空间和时间表达.
主要方法:
- 在玉米中分析miRNA166表达模式.
- 研究miRNA166和HD-ZIPIII基因之间的相互作用.
- 在体外切割试验.
主要成果:
- 微RNA166表达模式在空间上定义了玉米hd-zipIII基因,滚动叶1 (rld1) 的表达域.
- 在叶子发育过程中,miRNA166的表达逐渐扩大.
- miRNA166在叶膜中积累,这表明它可能是一个移动信号.
结论:
- 微RNA166是一种保存的偏振信号,对于确定叶子的轴向/轴向标识至关重要.
- miRNA166可以作为来自信号中心的移动信号分子发挥作用.
- 了解miRNA166的功能,可以了解植物的发育过程.
更多相关视频
相关概念视频
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

