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相关概念视频

Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...

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相关实验视频

Updated: May 10, 2026

Examining BCL-2 Family Function with Large Unilamellar Vesicles
08:35

Examining BCL-2 Family Function with Large Unilamellar Vesicles

Published on: October 5, 2012

在Bcl-2主要断点区域的非B-DNA结构被RAG复合体分裂.

Sathees C Raghavan1, Patrick C Swanson, Xiantuo Wu

  • 1Norris Comprehensive Cancer Center, Room 5428, University of Southern California Keck School of Medicine, 1441 Eastlake Ave., MC9176, Los Angeles, California 90033, USA.

Nature
|March 6, 2004
PubMed
概括

自发的染色体转位,像淋巴瘤中常见的t(14;18),通常是由DNA断裂引起的. 研究人员发现,Bcl-2基因中的非BDNA结构具有Bcl-2基因.

科学领域:

  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学
  • 癌症研究 癌症研究

背景情况:

  • 自发染色体转位在人类癌症中很常见,特别是在毛囊淋巴瘤中的t(14;18).
  • 导致Bcl-2基因主要断点区域 (Mbr) 断裂的确切机制尚不清楚.
  • 双链DNA断裂是所有拟议的转位机制的先决条件.

研究的目的:

  • 为了阐明Bcl-2 Mbr易受破碎的根本原因.
  • 研究DNA结构在染色体转位形成中的作用.
  • 了解RAG复合体如何与Bcl-2 Mbr.相互作用.

主要方法:

  • 在人体细胞中使用爱皮索姆系统复制转位特征.
  • 在体外和体内分离测定使用RAG复合物在Bcl-2 Mbr.
  • 在人类细胞和纯化DNA样本中分析DNA结构.

主要成果:

  • 该RAG复合体在体外和体内切断Bcl-2Mbr,反映转位模式.
  • 在20-30%的人类细胞等位基因中,Bcl-2 Mbr采用非B-DNA结构.
  • 这种非B-DNA结构表现出稳定的单链区域,对应于患者的转位部位.

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Visualization of DNA Repair Proteins Interaction by Immunofluorescence
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Visualization of DNA Repair Proteins Interaction by Immunofluorescence

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08:24

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Published on: July 27, 2021

相关实验视频

Last Updated: May 10, 2026

Examining BCL-2 Family Function with Large Unilamellar Vesicles
08:35

Examining BCL-2 Family Function with Large Unilamellar Vesicles

Published on: October 5, 2012

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion
08:24

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Published on: July 27, 2021

结论:

  • 在Bcl-2 Mbr的稳定非B-DNA结构是其基因组脆弱性的原因.
  • RAG复合体可以切割这种特定的非B-DNA结构,从而导致转位.
  • 这一发现为毛囊淋巴瘤中复发的t(14;18) 转位提供了一种机制.