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相关概念视频

Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Cross-reactivity00:42

Cross-reactivity

Overview
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Antibody Actions01:26

Antibody Actions

Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Production of Pharmaceuticals01:30

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...

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相关实验视频

Updated: Jul 10, 2026

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation
10:58

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Published on: August 21, 2019

在蛋白质酶体中通过合产生的抗原性.

Nathalie Vigneron1, Vincent Stroobant, Jacques Chapiro

  • 1Ludwig Institute for Cancer Research and Cellular Genetics Unit, Université de Louvain, B-1200 Brussels, Belgium.

Science (New York, N.Y.)
|March 6, 2004
PubMed
概括

CD8 T 淋巴细胞识别了由蛋白酶组拼接产生的黑色素瘤. 这种新的机制涉及连接不连续的蛋白质片段,以创建独特的抗原,用于免疫识别.

科学领域:

  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学
  • 癌症研究 癌症研究

背景情况:

  • CD8 T 淋巴细胞对于适应性免疫至关重要,它们识别由MHC I 类分子呈现的抗原.
  • 抗原呈现通常涉及从蛋白质降解中获得的线性.

研究的目的:

  • 调查黑色素瘤中CD8 T淋巴细胞识别的抗原的精确性质.
  • 在黑色素瘤的背景下阐明抗原产生的机制.

主要方法:

  • 对CD8 T淋巴细胞在黑色素瘤细胞上的酸识别的分析.
  • 在试验室中使用纯化的蛋白质酶复制生产.
  • 合机制的生物化学表征.

主要成果:

  • 在黑色素瘤细胞上识别了由CD8 T淋巴细胞识别的nonameric.
  • 这种质包括黑色细胞糖蛋白gp100 ((PMEL17) 的两个不连续的部分.
  • 证明蛋白酶可以切除氨基酸和拼接片段,这种过程可以在体外复制.

结论:

  • 黑色素瘤细胞呈现出从gp100(PMEL17) 到CD8 T淋巴细胞衍生出的一个拼接的nonameric.

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Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes
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Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
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Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium

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Last Updated: Jul 10, 2026

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation
10:58

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Published on: August 21, 2019

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13:53

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

Published on: December 19, 2020

Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
09:45

Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium

Published on: May 18, 2021

  • 蛋白质体在通过转化生成拼接的抗原中具有意想不到的功能.
  • 这一发现揭示了与癌症免疫学相关的抗原处理和T细胞识别的新途径.