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气味受体基因选择是通过从小鼠嗅觉感官神经元的核转移重置的
Jinsong Li1, Tomohiro Ishii, Paul Feinstein
1The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.
Nature
|March 26, 2004
概括
在小鼠嗅觉感官神经元中的嗅觉受体基因选择是不可逆转的,并且不受DNA重组的控制. 这种基因表达在核转移后被重置,没有发生基因组变化.
科学领域:
- 神经科学是一个神经科学.
- 遗传学 是一个遗传学.
- 嗅觉受体研究研究
背景情况:
- 哺乳动物基因组包含大约1000个嗅觉受体 (OR) 基因.
- 嗅觉感官神经元 (OSN) 通常表达来自一个等位基因的单个OR基因.
- 这种表达模式类似于淋巴细胞中的免疫球蛋白和T细胞受体基因调节.
研究的目的:
- 研究DNA重排是否控制OSN中的OR基因选择.
- 确定OR基因表达在OSN中的可逆性和基因组基础.
主要方法:
- 对表达M71的OSN进行永久基因标记.
- 核转移M71表达的OSN产生ntES细胞和克隆小鼠.
- 对ntES细胞系和衍生OSNs的DNA分析.
主要成果:
- 正如永久遗传标记所显示的那样,OSN中的M71 OR基因选择是不可逆转的.
- M71表达OSN的核转移导致ntES细胞和小鼠.
- DNA 分析显示,在衍生细胞系中,M71 位点没有发生重新排列或序列改变.
- 从ntES细胞分化出来的OSN可以表达M71.1.以外的OR基因.
结论:
- 在OSN中OR基因选择是不可逆转的,但不涉及DNA重排.
- 在核转移时,OR基因表达模式被重置,这表明最初选择的非基因组机制.
- 基因组变化不会伴随OSN中不可逆转的OR基因选择.
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