相关实验视频
Updated: Jul 20, 2026

09:36
Characterization of Anisotropic Leaky Mode Modulators for Holovideo
Published on: March 19, 2016
光热素的LOV2域:一个可逆的光色开关
John T M Kennis1, Ivo H M van Stokkum, Sean Crosson
1Department of Biophysics, Faculty of Sciences, Vrije Universiteit, 1081HV Amsterdam, The Netherlands. j.kennis@few.vu.nl
Journal of the American Chemical Society
|April 9, 2004
概括
光,氧或电压 (LOV) 域是蓝光敏感蛋白质. 超快光谱学揭示了LOV2域作为可逆光色开关,由蓝光激活,并被近紫外线光消灭.
科学领域:
- 摄影生物学 摄影生物学
- 蛋白质科学 蛋白质科学
- 生物物理学的生物物理.
背景情况:
- 光,氧或电压 (LOV) 域是蓝光光受体.
- 它们利用一种flavin染色体来提高光敏感度.
- 在蓝光吸收时,LOV2域形成了共价 adduct.
研究的目的:
- 为了研究LOV2域的共价 adduct 状态的光反应性.
- 确定引证债券改革的机制和时间表.
- 为了确定LOV2域的光色开关行为.
主要方法:
- 超快光谱学应用到LOV2.2的光积累的共价添加物.
- 研究了 adduct 状态对近紫外线光子的吸收.
- 对共价键动态的时间解析分析.
主要成果:
- 接近紫外线的光吸收会触发超快 (100 psi) 的共价键断裂.
- 蓝光敏感的基本状态很快恢复.
- 在LOV2域中展示可逆光色切换.
结论:
- 在LOV2域的功能作为一个可逆光色开关.
- 蓝光激活了交换机,通过形成一个共价 adduct.
- 接近紫外线的光线通过打破添加键来禁用开关.
相关概念视频
Photoelectric Effect
When light of a particular wavelength strikes a metal surface, electrons are emitted. This is called the photoelectric effect. The minimum frequency of light that can cause such emission of electrons is called the threshold frequency, which is specific to the metal. Light with a frequency lower than the threshold frequency, even if it is of high intensity, cannot initiate the emission of electrons. However, when the frequency is higher than the threshold value, the number of electrons ejected...
Channel Rhodopsins
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
UV–Vis Spectroscopy: Molecular Electronic Transitions
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...
Photoluminescence: Fluorescence and Phosphorescence
Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
A pair of electrons in a...
Photoluminescence: Applications
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Diode: Reverse bias
A diode is reverse-biased when the positive terminal of an external voltage source is connected to the n-type material and the negative terminal to the p-type material. This configuration opposes the natural direction of current flow through the diode, effectively increasing the width of the depletion region and the barrier potential. The reverse bias condition produces a minimal leakage current, primarily due to minority charge carriers. This leakage becomes significant when the reverse...

