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Ribozymes

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...

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面向高效的基于Zn(II) 的人工核酶.

Elisa Boseggia¹, Maddalena Gatos, Lorena Lucatello

¹Dipartimento di Scienze Chimiche e Istituto CNR Tecnologia delle Membrane-Sezione di Padova, Università di Padova, via Marzolo 1, I-35131 Padua, Italy.

Journal of the American Chemical Society

|April 9, 2004

概括

新的(II) 复合体-氨酸合物可以有效地切割等离子体DNA. 较长的基间隔器增强了DNA裂变,而8碳间隔器显示了最佳的活动.

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科学领域:

生物有机化学生物有机化学
化学生物学化学生物学
分子生物学分子生物学

背景情况:

DNA裂变剂对于分子生物学和治疗应用至关重要.
人类类衍生物是众所周知的DNA间隔器.
氨酸环素金属复合物可以充当催化中心.

研究的目的:

合成和评估新型 (II) 复合体 - - antraquinone 结合物作为水解性 DNA 裂变剂.
为了研究基间隔器长度对DNA裂变效率的影响.
了解这些DNA裂变系统中的结构-活性关系.

主要方法:

合成 cis-cis-triaminocyclohexane Zn(II) 复合体- antraquinone 结合体,其基间隔长度不同 (C4 到 C8).
在生理条件下 (pH 7,37°C) 使用超卷式DNA进行等离子体DNA裂变测定.
通过光谱光度测量对DNA裂变速率的监测.

主要成果:

这种C8结合的结合体在5μM度 (4.6 x 10^-6 s^-1) 时,显示了超绕DNA的显著的水解链裂变.
与 (II) 复合物与 antraquinone 的结合,与单独的 antraquinone 相比,将裂解效率提高了 15 倍.
随着间隔器的长度,DNA裂变效率增加,但由于位置不当,较短的间隔器导致了抑制.

结论:

(II) 复合物-氨酸合物是有效的水解性DNA裂变剂.
基间距长度对于优化DNA裂变活动至关重要,较长的间距通常会提高效率.
这些发现为针对性DNA裂变剂的设计提供了洞察力.