Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Citric Acid Cycle02:36

The Citric Acid Cycle

The citric acid cycle, also known as the Krebs cycle or TCA cycle, consists of several energy-generating reactions that yield one ATP molecule, three NADH molecules, one FADH2 molecule, and two CO2 molecules.
Loss of Carboxy Group as CO2: Decarboxylation of β-Ketoacids01:02

Loss of Carboxy Group as CO2: Decarboxylation of β-Ketoacids

Carboxylic acids, upon heating, undergo a decarboxylation reaction by releasing carbon dioxide gas. Monocarboxylic acids do not undergo decarboxylation easily. However, a silver salt of carboxylic acid reacts with bromine or iodine under high temperature to release carbon dioxide gas and forms halide with one less carbon. This reaction is called the Hunsdiecker reaction.
Microbial Fermentation01:23

Microbial Fermentation

Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
Amino Acid Catabolism01:18

Amino Acid Catabolism

Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
Carbon-dioxide Fixation01:28

Carbon-dioxide Fixation

Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
Production of Organic Acids01:25

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Evolution of Optimized Hydride Transfer Reaction and Overall Enzyme Turnover in Human Dihydrofolate Reductase.

Biochemistry·2021
Same author

Oscillatory Active-site Motions Correlate with Kinetic Isotope Effects in Formate Dehydrogenase.

ACS catalysis·2021
Same author

Caught in Action: X-ray Structure of Thymidylate Synthase with Noncovalent Intermediate Analog.

Biochemistry·2021
Same author

Rigid Double-Stranded DNA Linkers for Single Molecule Enzyme-Drug Interaction Measurements Using Molecular Recognition Force Spectroscopy.

Langmuir : the ACS journal of surfaces and colloids·2020
Same author

Isotopic Labeling of Formate Dehydrogenase Perturbs the Protein Dynamics.

The journal of physical chemistry. B·2019
Same author

Evolution Conserves the Network of Coupled Residues in Dihydrofolate Reductase.

Biochemistry·2019

相关实验视频

Updated: Jul 17, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

在大肠杆菌二酸盐还原酶催化过程中,道化和合运动.

R Steven Sikorski1, Lin Wang, Kelli A Markham

  • 1Department of Chemistry, University of Iowa, Iowa City, Iowa 52242, USA.

Journal of the American Chemical Society
|April 15, 2004
PubMed
概括

研究了二叶酸还原酶催化中的道化. 该研究发现了环境合道化,而不是合运动的证据,表明在稳定状态前反应期间对催化物的承诺.

科学领域:

  • 生物化学 生物化学
  • 酶动力学 酶动力学
  • 量子生物学就是量子生物学.

背景情况:

  • 二叶酸减少酶 (DHFR) 是叶酸代谢中的关键酶.
  • 了解DHFR中化物转移的机制是酶功能的关键.
  • 动力学研究对于阐明酶反应途径至关重要.

研究的目的:

  • 为了研究二叶酸还原酶动态级联中的转移机制.
  • 确定量子力学道在酶的催化过程中的作用.
  • 分析动力参数的温度依赖性,以了解反应动态.

主要方法:

  • 在二叶酸减少酶中研究了内在动态同位素效应 (KIEs).
  • 分析了KIE和其他动力参数的温度依赖性.
  • 使用了稳定状态前的运动分析.

主要成果:

  • 内在的动态同位素效应及其温度依赖性表明气道.
  • 没有发现结合1°至2°运动的证据.
  • 数据表明环境合道.
  • 致力于催化是从稳定状态前同位素效应推断出来的.

更多相关视频

Investigating the Alleviating Effects of Bacillus cereus Administration on Colitis through Gut Microbiota Modulation
08:34

Investigating the Alleviating Effects of Bacillus cereus Administration on Colitis through Gut Microbiota Modulation

Published on: July 27, 2022

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
10:23

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Published on: August 23, 2024

相关实验视频

Last Updated: Jul 17, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

Investigating the Alleviating Effects of Bacillus cereus Administration on Colitis through Gut Microbiota Modulation
08:34

Investigating the Alleviating Effects of Bacillus cereus Administration on Colitis through Gut Microbiota Modulation

Published on: July 27, 2022

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
10:23

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Published on: August 23, 2024

结论:

  • 在二叶酸减少酶中转移气可能涉及量子力学道化.
  • 道形成受到酶的环境的影响.
  • 酶对催化物的承诺发生在稳定状态前阶段.