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Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
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使用可变温度纳米电子喷雾质谱测量来确定蛋白质 - 配体关联热化学

Rambod Daneshfar1, Elena N Kitova, John S Klassen

  • 1Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.

Journal of the American Chemical Society
|April 15, 2004
PubMed
概括

一个新的温度控制的纳米电子喷雾装置与质谱学相结合,准确地测量了蛋白质-碳水化合物结合热力学. 这种方法提供了可靠的热力学数据,与已知技术 (如异热定位热量计) 相提并论.

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Heating a crystalline solid increases the average energy of its atoms, molecules, or ions, and the solid gets hotter. At some point, the added energy becomes large enough to partially overcome the forces holding the molecules or ions of the solid in their fixed positions, and the solid begins the process of transitioning to the liquid state or melting. At this point, the temperature of the solid stops rising, despite the continual input of heat, and it remains constant until all of the solid is...