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高发光兰坦化标签的工程适用于蛋白质标签和时间分辨率发光成像

Nicolas Weibel¹, Loïc J Charbonnière, Massimo Guardigli

¹Laboratoire de Chimie Moléculaire, Ecole de Chimie, Polymères et Matériaux, ULP, 25 rue Becquerel, 67087 Strasbourg Cedex 02, France.

Journal of the American Chemical Society

|April 15, 2004

概括

研究人员使用一种新型联结体开发了新的发光兰坦化物复合物. 这些复合物可以被附着在像BSA这样的生物分子上,用于生物分析应用,显示出高发光度和稳定性.

科学领域:

协调化学协调化学
生物有机化学生物有机化学
材料科学材料科学材料科学

背景情况:

新型连接体的开发对于创建功能金属复合体至关重要.
兰化物复合物为各种应用提供独特的发光特性.
生物结合策略对于将金属复合物与生物分子连接至关重要.

研究的目的:

基于谷氨酸骨架合成和表征一种新的配体,LH(4).
为了形成和研究与合成的联结体的胺 (III) 复合物.
评估这些复合物的生物结合和生物分析应用的潜力.

主要方法:

合成连接物LH(4) 和其类复合物 (Eu,Gd,Tb).
使用紫外线对光谱光度,红外线,核磁共振和质谱法进行表征.
确定稳定常数和发光特性 (量子产量).
复合物的共价连接到牛血清白蛋白 (BSA) 通过N-hydroxysuccinimidyl激活.
使用光谱学和MALDI-TOF质谱学对生物结合物的表征.

主要成果:

一个新型的配体LH(4) 已成功合成.
形成了稳定,水溶性1:1的兰化物复合物 ([LnL(H(2) O]Na).
高条件稳定常数 (log K = 16.5 +/- 0.6 对于 Eu).
对于水中的Eu (8%) 和Tb (31%) 复合物的高发光量子产量.
用Eu和Tb复合体成功标记BSA (标记比例为~8:1和~7:1).

结论:

合成的连接体形成稳定且高度发光的胺复合体.
碳酸盐组可以被激活为共价生物结合.
这些化物-BSA合物适用于生物分析应用,如时间分辨率发光显微镜.

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