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相关概念视频

Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...

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相关实验视频

Updated: Jul 18, 2026

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

核糖体蛋白S5的结构揭示了与16SrRNA相互作用的部位.

V Ramakrishnan1, S W White

  • 1Biology Department, Brookhaven National Laboratory, Upton, New York 11973.

Nature
|August 27, 1992
PubMed
概括

我们确定了第一个核糖体蛋白S5的晶体结构,这对蛋白质合成至关重要. 这种结构揭示了对转化忠实性和核糖体功能的洞察力,挑战了30S子单元的现有模型.

科学领域:

  • 分子生物学分子生物学
  • 结构生物学 结构生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 核糖体是必不可少的分子机器,负责将遗传密码转化为蛋白质.
  • 高分辨率的结构信息对于理解核糖体功能至关重要.
  • 核糖体蛋白S5是一种高度保存的蛋白质,存在于小核糖体子单元中.

研究的目的:

  • 确定核糖体蛋白S5.5的第一个晶体结构.
  • 调查S5.5的结构功能关系.
  • 分析S5突变如何影响核糖体功能和转化忠实度.

主要方法:

  • 使用X射线晶体学来确定S5.5的3D结构.
  • 对S5中的突变及其相关表型的分析.
  • 结构-功能分析,将S5结构与已知的模型进行比较.

主要成果:

  • S5的晶体结构显示了两个不同的α/β域,与其他核糖蛋白复合体组件有相似之处.
  • 在S5中发生的突变与表型有关,例如翻译模两可,链杆菌素依赖/耐药性和谱菌素耐药性.
  • 一种对寒冷敏感的突变体在发育过程中表现出缺陷,突变会映射到潜在的核糖体RNA相互作用部位.
  • 结构功能分析发现与30S核糖体子单元的当前模型存在差异.

更多相关视频

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
08:07

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

Published on: July 6, 2021

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
06:58

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

相关实验视频

Last Updated: Jul 18, 2026

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
08:07

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

Published on: July 6, 2021

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
06:58

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

结论:

  • 确定S5的结构为了解其在核糖体中的作用提供了基础.
  • S5可能在翻译忠实性和转位方面发挥着重要作用.
  • 这些发现挑战了30S核糖体子单元结构和功能的现有模型.