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生出可以发展到成年阶段的双遗传小鼠
Tomohiro Kono1, Yayoi Obata, Quiong Wu
1Department of BioScience, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan. tomohiro@nodai.ac.jp
Nature
|April 23, 2004
概括
由于基因组印记,哺乳动物不能通过伴生生殖通过无性繁殖. 研究人员通过操纵印制基因表达来创造了一个可行的基因小鼠,克服了这一障碍.
科学领域:
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 在哺乳动物中,没有观察到来自母亲的生殖细胞的无性繁殖,即帕氏生殖.
- 哺乳动物的发育通常需要母体和父体基因组,因为父母特异性的表观遗传修饰 (基因印记).
- 之前的研究表明基因组印记是代的障碍,但缺乏直接证据.
研究的目的:
- 调查基因组印记是否是防止哺乳动物 partenogenesis 的唯一障碍.
- 通过解决印制基因表达的方法,开发出一种可行的基遗传哺乳动物.
主要方法:
- 使用正常小鼠和被删除H19基因的小鼠的卵细胞创建了具有两个母体基因组组的重建卵细胞.
- 分析了印记基因的表达,包括Igf2和H19.
- 评估了部分遗传胚胎的发育进展和基因表达模式.
主要成果:
- 从重建的卵子细胞中发展到成年期的一只可行的伴生遗传小鼠.
- 这一发展与Igf2和H19等关键印记基因的适当表达有关.
- 异常基因表达在成功的parthenote中显著减少.
结论:
- 基因组印记,特别是父亲的贡献,是哺乳动物生的一个关键障碍.
- 克服印记基因失调可以使可活的基因哺乳动物的发展.
- 这项研究提供了直接的证据,表明父亲的基因组是哺乳动物发育的义务.
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