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相关概念视频

Electron Transport Chains01:28

Electron Transport Chains

The final stage of cellular respiration is oxidative phosphorylation that consists of two steps: the electron transport chain and chemiosmosis. The electron transport chain is a set of proteins found in the inner mitochondrial membrane in eukaryotic cells. Its primary function is to establish a proton gradient that can be used during chemiosmosis to produce ATP and generate electron carriers, such as NAD+ and FAD, that are used in glycolysis and the citric acid cycle.
The ETC is comprised of...
Catalysis02:50

Catalysis

The presence of a catalyst affects the rate of a chemical reaction. A catalyst is a substance that can increase the reaction rate without being consumed during the process. A basic comprehension of a catalysts’ role during chemical reactions can be understood from the concept of reaction mechanisms and energy diagrams.
Oxidative Cleavage of Alkenes: Ozonolysis01:46

Oxidative Cleavage of Alkenes: Ozonolysis

In ozonolysis, ozone is used to cleave a carbon–carbon double bond to form aldehydes and ketones, or carboxylic acids, depending on the work-up.
Ozone is a symmetrical bent molecule stabilized by a resonance structure.
Role of Reduced Coenzymes NADH and FADH₂01:29

Role of Reduced Coenzymes NADH and FADH₂

The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
Carbon-dioxide Fixation01:28

Carbon-dioxide Fixation

Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...

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相关实验视频

Updated: Jul 18, 2026

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

一氧化碳诱导的活性位点的分解 [Ni-4Fe-5S] 集群的CO脱酶.

Holger Dobbek1, Vitali Svetlitchnyi, Jago Liss

  • 1Laboratorium für Proteinkristallographie, Universität Bayreuth, Bayreuth, Germany. Holger.Dobbeck@uni-bayreuth.de

Journal of the American Chemical Society
|April 29, 2004
PubMed
概括

晶体结构揭示了一氧化碳脱酶 (CODHs) 内的Ni协调环境的差异. 该研究表明,CODHII中的[Ni-4Fe-4S]集群是来自活性[Ni-4Fe-5S]集群的非活性形式.

科学领域:

  • 生物化学 生物化学
  • 结构生物学 结构生物学
  • 酶学 是一种酶学.

背景情况:

  • 已经报告了含有Ni,Fe和S的各种一氧化碳脱酶 (CODH) 的晶体结构.
  • 厌氧细菌的CODHs具有一个集群C活性位点与Ni,Fe和S,显示不同物种的Ni协调环境的变化.
  • 之前的研究强调了蛋白质折叠的结构相似之处,但从*Carboxydothermus hydrogenoformans*,*Rhodospirillum rubrum*和*Moorella thermoacetica*的*CODHs的C集群内Ni协调的显著差异.

研究的目的:

  • 阐明 *Carboxydothermus hydrogenoformans* CODH II (CODHII(Ch) 中的 C 的结构与其蛋白质功能之间的关系.
  • 在不同的条件下研究CODHII的结构变化和它们对催化活性的影响.

主要方法:

  • 在不同的结晶条件下确定CODHII ((Ch) 的CO氧化活性.
  • 使用dithiothreitol或dithionite作为降解剂在N(2) 或CO大气下制备的酶晶体.
  • 在各种条件下,CODHII ((Ch) 的溶解晶体结构在1.1至1.6 Å的分辨率下.

主要成果:

  • 完全活跃的CODHII (((Ch) 在N (((2) 下与二化物化,显示了活跃的[Ni-4Fe-5S]集群.

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Solar-Driven Electrochemical Green Fuel Production from CO2 and Water Using Ti3C2Tx MXene-Supported CuZn and NiCo Catalysts
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Last Updated: Jul 18, 2026

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

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10:57

Synthesis and Performance Characterizations of Transition Metal Single Atom Catalyst for Electrochemical CO2 Reduction

Published on: April 10, 2018

Solar-Driven Electrochemical Green Fuel Production from CO2 and Water Using Ti3C2Tx MXene-Supported CuZn and NiCo Catalysts
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Solar-Driven Electrochemical Green Fuel Production from CO2 and Water Using Ti3C2Tx MXene-Supported CuZn and NiCo Catalysts

Published on: November 7, 2025

  • 短时间暴露于二氧化碳与二氧化二醇产生了具有催化能力的CODHII ((Ch) 与减少CO的[Ni-4Fe-5S]集群.
  • 长时间的CO处理导致活动丧失和形成一个缺少mu(2) -S连接体的非活性[Ni-4Fe-4S]集群,起源于[Ni-4Fe-5S]集群.

    • 结论:

    • 在CODHII中的[Ni-4Fe-4S]集群代表了活性[Ni-4Fe-5S]集群的无活化分解产物.
    • 在Ni协调环境中的结构差异显著影响CODH的功能.
    • 了解这些结构功能关系对于CODH酶机制至关重要.