光素为FRET提供了一个共振门,从合聚合物到DNA间染料

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Updated: Jul 11, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Shu Wang1, Brent S Gaylord, Guillermo C Bazan

  • 1Department of Chemistry, Institute for Polymers and Organic Solids, University of California, Santa Barbara, California 93106, USA.

Journal of the American Chemical Society
|April 29, 2004
PubMed
概括

这项研究引入了一种用于增强DNA传感的新型合聚合物. 聚合物,聚[1,4-]-2,7-[9,9-bis]6'-N,N,N-trimethylammonium) -hexyl]二氧化,通过级联能量转移机制提高了检测DNA结合染料的选择性.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...