核中的奥斯卡RNA的剪接与其细胞质局部化相结合
Olivier Hachet1, Anne Ephrussi
1European Molecular Biology Laboratory, Meyerhofstrasse 1, Postfach 10.2209, D-69117 Heidelberg, Germany.
Nature
|May 1, 2004
概括
在其第一个外因子-外因子结处合奥斯卡尔信使RNA (mRNA) 对于其在Drosophila卵细胞的后部定位至关重要. 这一发现揭示了剪接在调节发育过程中mRNA局部化的新作用.
科学领域:
- 发育生物学 发展生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 奥斯卡的mRNA定位到Drosophila卵细胞的后极对于胚胎发育至关重要.
- 核穿蛋白Y14/Tsunagi和Mago nashi,是人类外因子-外因子结合复合体 (EJC) 组件的同类,对于这种局部化是必需的.
研究的目的:
- 调查拼接在奥斯卡尔mRNA局部化中的作用.
- 为了质疑既定观点,即仅仅奥斯卡3'未翻译区域 (3'UTR) 就足以进行后部定位.
主要方法:
- 研究了在奥斯卡RNA的第一个前子-前子结处进行拼接的必要性,以实现后部定位.
- 通过检查不相关的转录的本地化,重新评估了奥斯卡尔3'UTR对后部定位的充分性.
主要成果:
- 在奥斯卡RNA的第一个前子-前子结处的剪接对于其后部定位至关重要.
- 含有oskar 3'UTR的转录的定位取决于内源的oskar mRNA,而不仅仅是3'UTR序列.
结论:
- 拼接在调节mRNA局部化方面起着至关重要的,以前未被认可的作用.
- 拼接对于细胞质mRNA定位的信使核糖核蛋白复合体的组装和组织至关重要.
相关概念视频
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Regulated mRNA Transport
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Regulated mRNA Transport
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...


