高效的光化学2'-脱氧里博诺拉克在含有5 - आयो多拉的反平行G四重奏的对角环上形成
1Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda, Tokyo 101-0062, Japan.
Journal of the American Chemical Society
|May 20, 2004
概括
这项研究揭示,在反平行G-四重奏DNA结构中,5-多拉特别形成2'-脱氧里博诺拉克. 这种发生在对角环中的光化学反应可以作为这些DNA结构的探测器.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 摄影化学的使用.
背景情况:
- G四重奏是具有多种生物作用的四链DNA结构.
- 了解它们的结构依赖反应性对于生物学见解至关重要.
- 5 - 多 ((I) U) 是DNA研究中有用的光化学探针.
研究的目的:
- 为了研究反平行和平行G-四重奏DNA结构的结构依赖光化学反应性.
- 探索在紫外线照射下在含有 (I) U的DNA中2 - - 脱氧里博诺拉克的形成.
- 评估这种反应作为反平行G四重奏的特定探针的潜力.
主要方法:
- 紫外线照射 (302nm) 含有5-欧拉的人类端粒DNA22-mers.
- 对光化学反应产物的分析,特别是2 - 脱氧里博诺拉克的形成.
- 反平行与平行G四重奏和其他DNA形式中的反应性比较.
主要成果:
- 反平行G四重奏ODN 4与 (I) U在对角环被迅速消耗在紫外线照射.
- 2 -deoxyribonolactone是在反平行G四重奏的对角环中 (I) U残留的5'侧形成的.
- 在平行G四重奏,单链DNA或发针结构中没有观察到这种反应.
- 在IgG切换区域和Rb基因的G丰富序列中也检测到2-脱氧里博诺拉克的形成.
结论:
- 2 - - 脱氧里博诺拉克的光化学形成对抗平行G四重奏的对角循环具有高度的特异性.
- 这种反应可以用作特定的光化学探针来检测具有对角环的反平行G四重奏.
- 这些发现表明,在生物相关的富G序列中存在这样的结构.
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