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Viral Structure

Viral Structure

Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.

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一个量子点结合糖球及其细胞吸收. 关于在亚病毒区域内内细胞结核的尺寸效应.

Fumio Osaki¹, Takuya Kanamori, Shinsuke Sando

¹Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.

Journal of the American Chemical Society

|May 27, 2004

概括

此摘要是机器生成的。

这项研究表明,化物量子点 (CdSe QDs) 可以在细胞入侵中具有水溶性. 最佳的纳米颗粒大小,大约50纳米,对于通过内细胞分裂有效的细胞吸收至关重要.

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科学领域:

纳米技术纳米技术
生物医学工程生物医学工程
细胞生物学细胞生物学

背景情况:

用三氧化物 (TOPOQD) 涂覆的性化物量子点 (CdSe QD) 不溶于水.
开发水溶性纳米粒子对于生物医学应用至关重要,包括药物和基因传递.
了解纳米粒子-细胞相互作用,特别是内细胞分裂,是设计有效传递系统的关键.

研究的目的:

开发一种方法,使脂性CdSe QDs具有水溶性.
研究水溶性CdSe QDs的细胞吸收机制和效率.
为了确定纳米颗粒大小对细胞通过内细胞分裂吸收的影响.

主要方法:

通过使用宏循环葡萄糖集群两的方法从甲中提取TOPOQD到水中.
将TOPOQD与两生物结合,形成一个光纳米粒子 (TOPOQD1).
评估细胞吸收TOPOQD1在Hela细胞使用内细胞结核和与不同尺寸的参考纳米颗粒进行比较.

主要成果:

成功使脂友性CdSe QDs溶于水 (TOPOQD1,15 nm) 成为可溶性.
TOPOQD1通过内细胞分裂有效地侵入Hela细胞.
证明了对内细胞活动的显著尺寸依赖作用,最佳吸收在50nm左右,揭示了尺寸互补原则.

结论:

纳米颗粒的尺寸控制对于由内细胞囊泡有效封装细胞至关重要.
纳米粒子大小在分子识别和传递中起着至关重要的作用,类似于宿主-客户系统.
设计有效的分子输送机器需要精确的尺寸优化,特别是在亚病毒到病毒尺寸范围内.