氨酸激酶II是c-Jun DNA结合和AP-1活动的负调节者
1Department of Pharmacology, University of California, San Diego School of Medicine, La Jolla 92093-0636.
Cell
|September 4, 1992
概括
凯激酶II (CKII) 酸化c-Jun,抑制其DNA结合和AP-1活性. 这项研究揭示了CKIIII.
科学领域:
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
- 生物化学 生物化学
背景情况:
- c-Jun是AP-1转录因子的关键组成部分,调节基因表达.
- 化c-Jun抑制其DNA结合活性,影响AP-1的转录功能.
- 负责c-Jun酸化的特定激酶及其调节作用尚未完全理解.
研究的目的:
- 在c-Jun.上确定负责酸化抑制位点的激酶.
- 阐明素激酶II (CKII) 在调节AP-1活性中的作用.
- 研究CKII介导c-Jun的酸化如何影响AP-1的转录功能.
主要方法:
- 在c-Jun酸化位点的位点定向突变发生.
- 在体外激酶试验中使用纯化素激酶II.
- 在体内研究涉及微注射和CKII到细胞.
- 分析AP-1活动和c-Jun表达水平.
主要成果:
- 凯激酶II (CKII) 在6月4日被确定为酸化Thr-231和Ser-249的激酶.
- 将Ser-243替换为氨,通过CKII.II损害了c-Jun酸化.
- 微注射CKII抑制剂诱导AP-1活动和c-Jun表达.
- 通过醇或抑制性的微注射抑制了CKII的AP-1诱导.
结论:
- 素激酶II (CKII) 通过酸化c-Jun. 在减弱AP-1活性方面发挥着至关重要的作用.
- CKII作为AP-1转录活动的负调节剂.
- 这些发现揭示了CKII在细胞信号通路中的新功能.
更多相关视频
09:52A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
Published on: December 4, 2018
11:32Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
Published on: March 27, 2020
相关概念视频
RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Eukaryotic Transcription Activators
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
