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Updated: Jul 8, 2026

Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers
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Published on: June 3, 2019

对哺乳动物中体蛋白质组的剖析揭示了保存的细胞运动机制

Ahna R Skop1, Hongbin Liu, John Yates

  • 1Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA. skop@wisc.edu

Science (New York, N.Y.)
|May 29, 2004
PubMed
概括

科学家使用先进的蛋白质和基因组方法确定了参与细胞分裂 (细胞动力学) 的关键蛋白质. 许多这些蛋白质对于适当的细胞分裂和在各种细胞类型中的功能至关重要.

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Mechanical Protein Function01:58

Mechanical Protein Function

Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...