酶催化生物电子到au-纳米粒子:一个表面等离子体共振和电化学研究
Oleg Lioubashevski1, Vladimir I Chegel, Fernando Patolsky
1Institute of Chemistry, The Farkas Center for Light-induced Processes, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Journal of the American Chemical Society
|June 4, 2004
概括
在金纳米颗粒上复制的葡萄糖氧化酶产生电信号,可通过表面等离子体共振检测到. 这种生物催化充电现象与葡萄糖度相关,使潜在的生物感知应用成为可能.
科学领域:
- 纳米技术纳米技术
- 生物化学 生物化学
- 电化学 电化学 电化学
背景情况:
- 对纳米材料的酶固定对于生物传感器的发展至关重要.
- 酶和导电表面之间的电通信是信号转导的关键.
- 表面等离子共振 (SPR) 是监测界面变化的敏感技术.
研究的目的:
- 在氨酸腺素二核酸 (FAD) 功能化黄金纳米粒子 (Au-NPs) 上复制葡萄糖氧化酶 (GOx).
- 调查GOx氧化还原辅因子和Au-NPs之间的电气通信.
- 建立基于SPR检测生物催化性充电的葡萄糖传感平台.
主要方法:
- 在FAD功能化的Au-NPs (1.4 nm) 上复制GOx.
- 在金电极上的GOx/Au-NP杂交物通过短 (1,4-甲基乙醇) 或长 (1,9-非甲基乙醇) 甲基乙醇单层固定.
- 监测由于Au-NP充电而导致的SPR光谱 (等离子体角,theta(P)) 的变化.
- 使用差电容和电极电位测量进行表征.
主要成果:
- 在GOx辅因子和Au-NP核心之间证明了电气通信.
- 葡萄糖被GOx生物催化氧化导致Au-NP充电,改变了SPR光谱.
- 与葡萄糖度相关的SPR转移 (theta(P)) 与葡萄糖度相关.
- 每个Au-NP储存的估计电子在不同的葡萄糖度和连接器长度.
结论:
- 该GOx/Au-NP混合系统通过生物催化充电实现了基于SPR的葡萄糖检测.
- 醇连接器长度影响电子道化和SPR反应.
- 这种方法提供了一种用于电化学传感和量化纳米材料中电子储存的新方法.
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