在胚胎神经元中发射器表达的活动依赖的恒常规范
Laura N Borodinsky1, Cory M Root, Julia A Cronin
1Neurobiology Section, Division of Biological Sciences and Center for Molecular Genetics, UCSD, La Jolla, California 92093-0357, USA. lborodin@biomail.ucsd.edu
Nature
|June 4, 2004
概括
在发育中的脊髓神经元中改变神经元活动模式会改变神经递质表达,而不会改变细胞身份. 这种恒常性调节表明,有模式的活动对中枢神经系统发育至关重要.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 细胞信号传输 细胞信号传输
背景情况:
- 神经递质对神经元通信至关重要.
- 神经发射器规范与内在的身份和外在的信号有关.
- 显著的Ca2+峰值活动模式是胚胎脊髓神经元类的特征.
研究的目的:
- 研究Ca2+峰值活动在确定神经递质在发育中的神经元表达中的作用.
- 为了确定神经元活动是否影响发射器选择,独立于细胞身份标记.
主要方法:
- 在体内操纵胚胎脊髓神经元中自发的Ca2+峰值活动.
- 在实验室中强加特定的Ca2+峰值频率.
- 对神经递质表达和细胞身份标记物的分析.
主要成果:
- 改变Ca2+峰值活动模式改变了神经递质表达,但没有改变细胞身份标记.
- 活动抑制增加了刺激性和降低了抑制性传递器表达.
- 增强的活性显示出相反的效果,表明恒常性调节.
- 在临床实验室活动模式中,在关键的发育时期指定了不合适的发射器.
结论:
- 模式神经元活动在发育过程中在确定神经递质表型方面发挥着关键作用.
- 这种活动依赖的调节似乎是恒常的.
- 确定了模式活动在中枢神经系统发育中的新角色.
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