需要进行基因间转录来抑制Saccharomyces cerevisiae SER3基因
Joseph A Martens1, Lisa Laprade, Fred Winston
1Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA.
Nature
|June 4, 2004
概括
基因间转录产生非编码RNA (SRG1),抑制酵母中的SER3基因表达. 这种SRG1RNA通过转录干扰作用,揭示了一个新的调节机制.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 基因规则 基因规则
背景情况:
- 广泛的RNA聚合酶II转录发生在酵母和人类的非蛋白质编码基因组区域.
- 这种基因间转录的功能在很大程度上是未知的,但可能发挥调节作用.
研究的目的:
- 用Saccharomyces cerevisiae SER3基因作为模型研究基因间转录的功能.
- 阐明 SER3 转录在丰富介质中被抑制的机制.
主要方法:
- 对Saccharomyces cerevisiae中的基因表达的分析.
- 检测和量化RNA的检测和量化.
- 对转录干扰机制的研究.
主要成果:
- 一种非蛋白质编码RNA,SRG1,从丰富的介质中转录出SER3基因的调节区域.
- 对于抑制SER3转录,SRG1RNA表达是必不可少的.
- SRG1转录干扰了转录激活剂与SER3促进体的结合.
结论:
- 确定了一种涉及基因沉默的新型转录调节基因类.
- 通过转录干扰通过非编码RNA调解的基因抑制机制.
- 突出了以前未经表征的基因间转录的调节潜力.
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