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Updated: Jul 3, 2026

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell
14:09

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Published on: August 4, 2015

同时触发蛋白质活性和光

Jean-Philippe Pellois1, Michael E Hahn, Tom W Muir

  • 1Laboratory of Synthetic Protein Chemistry, The Rockefeller University, New York, New York 10021, USA.

Journal of the American Chemical Society
|June 10, 2004
PubMed
概括

研究人员开发了一种新的方法来控制使用光的蛋白质活性和光. 该技术允许精确监测蛋白质功能,特别是转化生长因子-β (TGF-β) 信号通路中的蛋白质功能.

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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...