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An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation05:44

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Here we present a method to express acetylated histone proteins using genetic code expansion and assemble reconstituted nucleosomes in...
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The purpose of this article is to provide a comprehensive, systematic guide to the efficient purification of histones H3 and H4 and the quantification of acetylated histone...
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Histone Modification02:32

Histone Modification

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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
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相关实验视频

Updated: Jan 19, 2026

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening
05:44

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening

Published on: January 26, 2024

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将全球基因素乙化模式映射到基因表达.

Siavash K Kurdistani1, Saeed Tavazoie, Michael Grunstein

  • 1Department of Biological Chemistry, UCLA School of Medicine and the Molecular Biology Institute, Boyer Hall, Los Angeles, CA 90095, USA.

Cell
|June 10, 2004
PubMed
概括

酵母菌中核心基因素上的素乙化模式揭示了不同的基因组. 这些模式与基因活性,共同表达和转录因子结合相关,为染色质调节提供了洞察力.

科学领域:

  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 基因组学就是基因组学.

背景情况:

  • 基因组乙转移酶 (HAT) 和脱乙酶 (HDAC) 通过修改基因组乙化来调节基因表达.
  • 基因质上特定的乙化位点可以产生独特的模式,影响生物过程.
  • 了解这些模式对于破译基因调节机制至关重要.

研究的目的:

  • 为了研究Saccharomyces cerevisiae中核心基因素的全基因组乙化概况.
  • 为了确定特定的 lysine 乙化模式与基因活性之间的关系.
  • 为了确定不同的乙化模式是否定义生物学相关的基因组.

主要方法:

  • 针对四个核心基因组中的11个lysines的乙化概况的全基因组分析.
  • 乙化状态和基因转录水平之间的相关性分析.
  • 共同表达的基因的识别,共享的cis调节动机,以及转录因子结合丰富.

主要成果:

  • 单个溶酶的过度和低乙化与基因转录有关.
  • 独特的乙化模式定义了生物相关和共同表达的基因组.
  • 这些基因组共享cis调节动机,并为特定的转录因子结合而丰富,包括Bdf1,在H4 lysine 16上有一个显著的例外.

更多相关视频

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
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Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
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相关实验视频

Last Updated: Jan 19, 2026

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening
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Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening

Published on: January 26, 2024

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Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
07:26

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Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
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Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

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结论:

  • 特定的基因组乙化模式作为生物相关基因的调节机制.
  • 这些模式可以指定蛋白质 - 基因素相互作用,促进协调的染色质调节.
  • 这些发现为了解乙化模式如何影响基因表达和细胞过程提供了一个框架.