在单一树突状棘中长期增强的结构基础
Masanori Matsuzaki1, Naoki Honkura, Graham C R Ellis-Davies
1Department of Cell Physiology, National Institute for Physiological Sciences and The Graduate University of Advanced Studies (Sokendai), Myodaiji, Okazaki 444-8787, Japan.
Nature
|June 11, 2004
概括
状脊柱结构随活动而变化,形成学习和记忆的基础. 小棘是长期增强力量的关键,而大棘可能会存储长期记忆.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 突触性可塑性 突触性可塑性
背景情况:
- 金字塔神经元中的状棘经历了活动依赖的结构重塑,这是学习和记忆的细胞基础.
- 结构重塑支持突触可塑性的确切机制,如长期强化 (LTP),以及其在个体脊柱层面的输入特异性仍然不清楚.
研究的目的:
- 调查长期增强 (LTP) 在单一树突脊柱水平的结构基础.
- 为了确定突触可塑性是否在单个脊柱的输入特定.
主要方法:
- 利用格谷氨酸的双光子光解来诱导海马体CA1金字塔神经元单个脊柱的可塑性.
- 检查的结构变化和相关的突触电流变化,以应对受控的谷氨酸脱.
主要成果:
- 重复的谷氨酸脱诱发了快速的,选择性的脊柱扩大.
- 扩大在大的棘中是暂时的,但在小的棘中是持续的.
- 脊柱扩大与AMPA受体电流的增加相关,并依赖于NMDA受体,卡尔莫杜林和活性蛋白聚合. 需要长期扩大Ca2+/卡尔莫杜林依赖蛋白激酶II.
结论:
- 个别的树突脊柱坚持赫布的学习假设.
- 小棘似乎是诱导长期增强潜力的首选地点.
- 大的棘可以作为长期记忆痕迹的物理存储库.
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