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用于检测DNA和端粒酶活动的催化信标

Yi Xiao1, Valeri Pavlov, Tamara Niazov

  • 1The Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

Journal of the American Chemical Society
|June 17, 2004
PubMed
概括

一种新的DNA酶检测DNA和端粒酶活性. 这种催化信标系统为分子检测和诊断提供了一种新方法.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...