双脂质的分割成膜微域:脂质分类与聚合
Sascha Janosch1, Chiara Nicolini, Björn Ludolph
1Department of Chemistry, Physical Chemistry I, University of Dortmund, Otto-Hahn-Strasse 6, D-44227 Dortmund, Germany.
Journal of the American Chemical Society
|June 17, 2004
概括
这项研究表明,双脂质如何与细胞膜相互作用. 这些可以诱导流体微域,特别是与胆固醇,影响膜组织和定位.
科学领域:
- 生物物理学的生物物理.
- 膜生物学 膜生物学
- 利皮多米克 (Lipidomics) 是一种消化剂.
背景情况:
- 细胞膜表现出复杂的横向组织和相位行为.
- 拉斯蛋白对于细胞信号传递至关重要,与特定的膜域有关.
- 棕基/法尼西尔双脂质作为Ras蛋白膜相互作用的模型系统.
研究的目的:
- 为了研究带有和没有Ras模型的脂质混合物的侧面膜组织和相位行为.
- 了解胆固醇如何影响膜结构和位定位.
- 阐明-膜相互作用和域形成的机制.
主要方法:
- 两光子激发光显微镜被用来研究巨型单囊泡 (GUVs).
- 对DMPC,DSPC和胆固醇的脂质混合物进行了广泛的温度范围 (30-80°C) 的分析.
- 一种光标记的棕/法纳双脂质被纳入了脂质系统.
主要成果:
- 胆固醇的结合增加了膜秩序,减少了大域结构.
- 诱导相位分离,形成富含的液体微域,特别是在胆固醇的存在下.
- 的局部化高度依赖于脂质组成和温度,有利于液体而不是有序或凝域.
- 在低温下,由于脂质分类机制失败,聚成小发生了.
结论:
- 棕醇/法内西尔双脂质酸与液体排序或凝状膜域没有关联.
- 这些可以在生理上相关的胆固醇度下诱导流体微域的形成.
- 的形成微域的能力取决于膜中的液体和有序脂质成分的平衡.
相关概念视频
01:38What are Lipids?
Overview
03:38Structure of Lipids
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic birds and...
03:38Structure of Lipids
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic birds and...
01:32Membrane Lipids
Lipids are an essential component of all biological membranes. The average lipid content in mammalian membranes is 50%, though it can be as low as 20% in the inner mitochondrial membrane or as high as 80% in the myelin sheath present around the nerve cells.
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
03:38Structure of Lipids
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic birds and...
01:32Membrane Lipids
Lipids are an essential component of all biological membranes. The average lipid content in mammalian membranes is 50%, though it can be as low as 20% in the inner mitochondrial membrane or as high as 80% in the myelin sheath present around the nerve cells.
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...