In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

Brick masonry uses bricks as the building blocks and involves building walls from individual bricks laid in mortar. The basic building block of brick masonry is the wythe, a vertical layer of bricks with a thickness of one brick. Within a wythe, bricks can be laid in various courses or patterns, with the most common being the stretcher course, where bricks are laid with their long edge horizontal and face parallel to the wall.
For thicker walls, multiple wythes are bonded together using...

Mortar joints play a critical role in brick masonry, filling the spaces between brick to bind them together and provide structural integrity and strength. The thickness of these joints is variable, typically ranging from less than one-fourth inch to over half an inch, based on structural needs and specific applications.
The process of joint tooling is implemented as the mortar begins to harden. This technique involves compacting and shaping the mortar to enhance both the appearance and the...

Building stones, essential materials for construction, are extracted from natural rock deposits and processed into specific forms and dimensions suitable for various building applications. These stones are broadly classified into three types based on their geological formation: igneous, sedimentary, and metamorphic.
Igneous rocks are formed from the solidification of magma or lava. An example is granite, known for its durability and resistance to weathering, making it ideal for parts of...

Quarrying is the process of extracting stone from a quarry, where specialized techniques are employed to remove large blocks of stone safely and efficiently. This process can involve controlled explosions or more precision-oriented methods such as cutting and drilling.
One common method involves using a diamond belt saw to cut large blocks from the quarry face. These blocks can be about 50 feet long and 12 feet high. After the initial vertical cut, drilling is performed at the base of the block.

Stone masonry is a construction technique that uses individual stones to build structures and can be categorized into two main types: rubble and ashlar. Rubble masonry uses uneven, naturally shaped stones such as river rocks or fragments from quarries. This method often requires the mason to select and possibly shape each stone to fit the designated space, ensuring a proper build, even with irregular stone sizes and shapes. Ashlar masonry, on the other hand, employs uniformly cut stones that...