胚胎细胞植入取决于母体白血病抑制因子的表达
C L Stewart1, P Kaspar, L J Brunet
1Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
Nature
|September 3, 1992
概括
白血病抑制因子 (LIF) 对于小鼠的胚胎细胞植入至关重要. 没有功能性LIF,胚胎无法植入,突出其在怀孕早期的重要作用.
科学领域:
- 生殖生物学 生殖生物学
- 发育生物学是发展生物学.
- 分子信号传递是分子信号传递.
背景情况:
- 哺乳动物怀孕涉及囊胚植入,胚胎附着在子宫壁上.
- 这个过程是由母亲和胚胎之间复杂的荷尔蒙相互作用同步的.
- 精确的控制机制,母体与胚胎,控制植入,仍然在很大程度上是未知的.
研究的目的:
- 为了研究白血病抑制因子 (LIF) 在胚胎细胞植入中的作用.
- 确定LIF表达是否对于成功的胚胎植入和发育至关重要.
主要方法:
- 在怀孕期间对子宫内膜中LIF表达的分析.
- 产生和研究缺乏功能性LIF基因的小鼠.
- 在LIF缺乏和野生类型小鼠中的胚胎细胞植入和发育的评估.
- 在LIF缺陷和野生类型接受者之间进行胚胎细胞移植实验.
主要成果:
- 白血病抑制因子 (LIF) 在子宫内膜中短暂表达,特别是在怀孕的第四天,在胚胎细胞植入之前.
- 缺乏功能性LIF基因的雌性小鼠是肥沃的,但它们的囊胚细胞无法植入.
- 这些非植入的胚胎细胞是可行的,可以成功地植入和发展到期,当转移到伪怀孕的野生类型的接受者.
结论:
- 白血病抑制因子 (LIF) 的短暂表达对于在小鼠中成功植入胚胎细胞至关重要.
- LIF以母亲的方式为胚胎植入做准备,为子宫环境做准备.
- 这项研究确定LIF是启动怀孕所需的母胚对话的关键调节者.
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