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High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
Published on: October 15, 2019
胰腺脂酶 - - 蛋白脂酶复合体的结构
H van Tilbeurgh1, L Sarda, R Verger
1LCCMB-CNRS, Faculté de Médecine Nord, Marseille, France.
Nature
|September 10, 1992
概括
胰腺脂酶 - - 蛋白酶复合物的结构显示,蛋白酶具有
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 酶学 是一种酶学.
背景情况:
- 胰腺脂酶对脂质接口的吸附受到脂和胆汁盐等两性生物的影响.
- 脂酶结合需要在这些两性动物的存在下使用辅因子酶.
研究的目的:
- 为了阐明胰腺脂酶-前脂酶复合体的三维结构.
- 了解胆酶与胰腺脂酶相互作用的结构基础.
主要方法:
- 胰腺脂酶 - - 蛋白酶复合物的结晶.
- 通过X射线晶体学,以3.04A分辨率解决结构.
主要成果:
- 益生菌酶的结构由三个"手指"组成,在拓上与蛇毒素相似.
- 普罗科利帕斯的疏水氨基酸位于尖端,形成假定的界面结合部位.
- 益生菌酶通过极性相互作用与胰腺脂酶的C端域结合,而不会诱导脂酶的形状变化.
结论:
- 该结构提供了对脂酶界面结合和酶功能机制的见解.
- 益生菌酶作为胰腺脂酶的C端域的特定结合伙伴.
- 在胆汁盐的存在下,脂酶-合酶相互作用对于有效的脂肪消化至关重要.
相关概念视频
Protein Organization
Overview
Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Protein and Protein Structure
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
A protein's shape is critical to its function. For example, an enzyme can...
Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Protein Organization
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
The primary structure of a protein is its amino acid sequence.

