在cis和trans基因调节中的进化变化.
Patricia J Wittkopp1, Belinda K Haerum, Andrew G Clark
1Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA. pw72@cornell.edu
Nature
|July 2, 2004
概括
基因表达的差异推动了进化. 这项研究表明,大多数进化基因表达变化源于多种cis-regulatory变化,而不是Drosophila物种之间广泛的跨调节效应.
科学领域:
- 进化生物学是进化的生物学.
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 基因表达的差异是进化过程的基础.
- 无论是 cis-regulatory 和 trans-regulatory 的变化都会导致基因表达的分歧.
- 对于 cis 和 trans 调控变化对表达差异的相对贡献尚不清楚.
研究的目的:
- 研究Drosophila melanogaster和Drosophila simulans之间基因表达差异的基础的cis和跨调节性变化的分布.
- 量化 cis 和 trans 调控变化对特定物种基因表达分歧的影响.
主要方法:
- 在F1杂交物种中比较物种特异性转录的相对丰度,以确定功能性 cis-regulatory 差异.
- 通过将杂交物中的等位基因表达比率与跨种类基因表达比率进行比较,推断跨调节活性.
主要成果:
- 在29个具有跨物种表达差异的基因中,28个显示出 cis 调节性变化.
- 单独的Cis调节变化就解释了大约一半研究的基因的表达分歧.
- 在55%的基因中观察到跨调节差异,并且总是与cis调节变化同时发生.
结论:
- 各种基因表达之间的差异主要是由众多的基因组范围内的cis作用调节变化驱动的.
- 具有广泛影响的广泛的跨调节变化并不是这些多索菲拉物种表达差异的主要原因.
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