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Updated: Jul 21, 2026

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
13:59

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins

Published on: December 12, 2013

尿素脱化蛋白中的残留结构的NMR确定,即434抑制剂

D Neri1, M Billeter, G Wider

  • 1Institut für Molekularbiologie und Biophysik, ETH-Hönggerberg, Zürich, Switzerland.

Science (New York, N.Y.)
|September 11, 1992
PubMed
概括

这项研究确定了在去化尿素溶液中的蛋白质域的核磁共振 (NMR) 结构. 鉴定出由特定残留物 (54-59) 形成的疏水性集群,为蛋白质重新折叠提供了洞察力.

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¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...