糖化对构成的影响:一个协同的实验和计算研究
Carlos J Bosques1, Sarah M Tschampel, Robert J Woods
1Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Journal of the American Chemical Society
|July 9, 2004
概括
碳水化合物-链的立体化学反应极大地影响了糖结构. 这项研究揭示了阿尔法和β结合的糖化物如何独特地影响和糖的构造.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 葡萄糖生物学 葡萄糖生物学
背景情况:
- 与阿斯巴拉金结合的糖化对蛋白质折叠,稳定性和功能至关重要.
- 糖蛋白碳水化合物组成是异质的,但核心二糖化物 (β-D-(GlcNAc) 2) 被保存.
- 之前的研究表明,核心二糖化物独特地影响了的脊柱形状.
研究的目的:
- 调查碳水化合物-酸链接立体化学对糖结构的影响.
- 分析阿尔法和β链接如何影响糖的构造.
主要方法:
- 合成了由血凝素衍生的糖和一个与α相关的碳水化合物.
- 采用了协同的实验和计算方法.
- 利用计算和生物物理分析.
主要成果:
- 碳水化合物-链的立体化学显著影响糖的构成.
- 两种α-和β-链接的糖都表现出独特的形状特征.
- 计算方法有效地探测了糖胺对多结构的影响.
结论:
- 碳水化合物-酸链接立体化学是糖结构的关键决定因素.
- 附着的糖化物独特地调节了和糖的构造.
- 计算方法对于理解糖化对蛋白质结构的影响是有价值的.
相关概念视频
02:56Formula Mass and Mole Concepts of Compounds
Formula Mass of Covalent Compounds
03:25Chemistry of Carbohydrates
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
03:25Chemistry of Carbohydrates
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
01:10Sugars as Energy Storage Molecules
Sugar (a simple carbohydrate) metabolism (chemical reactions) is a classic example of the many cellular processes that use and produce energy. Living things consume sugar as a major energy source because sugar molecules have considerable energy stored within their bonds. Consumed carbohydrates have their origins in photosynthesizing organisms like plants. During photosynthesis, plants use the energy of sunlight to convert carbon dioxide gas into sugar molecules, like glucose. Because this...
03:25Chemistry of Carbohydrates
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
01:10Sugars as Energy Storage Molecules
Sugar (a simple carbohydrate) metabolism (chemical reactions) is a classic example of the many cellular processes that use and produce energy. Living things consume sugar as a major energy source because sugar molecules have considerable energy stored within their bonds. Consumed carbohydrates have their origins in photosynthesizing organisms like plants. During photosynthesis, plants use the energy of sunlight to convert carbon dioxide gas into sugar molecules, like glucose. Because this...