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将具有多系潜力的胎儿细胞转移到母体组织中

Kiarash Khosrotehrani¹, Kirby L Johnson, Dong Hyun Cha

¹Department of Pediatrics, Tufts-New England Medical Center, Boston, Mass 02111, USA.

|July 9, 2004

概括

持续存在于母亲的胎儿细胞可以分化为各种细胞类型,包括上皮细胞,白细胞和肝细胞. 这项研究证实,胎儿微化学细胞在母体器官中表现出多个血统的潜力.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
遗传学是一个遗传学.

背景情况:

胎儿细胞 (CD34+) 在怀孕期间进入母亲的血液循环,建立长期的微化学反应.
在怀孕后的母亲血液和组织中发现了残留的胎儿细胞.
胎儿细胞可能在母体器官中分化为多个血统,以应对损伤.

研究的目的:

确认胎儿微化学细胞表达表皮细胞,白细胞和肝细胞在母体器官的分化标记.
在母体环境中研究胎儿细胞的多系潜力.

主要方法:

用XY染色体的光在位杂交 (FISH) 来分析具有男性后代的女性的母体组织样本.
免疫组织化学染色用于细胞卡素 (表皮),CD45 (白细胞) 和heppar-1 (肝细胞) 标记物.
对表达特定分化标记的微化学细胞的量化.

主要成果:

鉴定了701个雄性微化学细胞 (XY+).
14%-60%的XY+细胞在母亲的上皮组织表达细胞克拉丁.
在造血组织中,90%的XY+细胞表达了CD45;在肝组织中,4%的细胞表达了heppar-1.

结论:

在母体组织中检测具有上皮细胞,白细胞和肝细胞标记物的胎儿微化学细胞.
表明这些胎儿细胞在母体器官内具有多系分化能力.

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