使用支的液体膜封装表面活性剂-酶复合物的高度酶选择性分离
Eijiro Miyako1, Tatsuo Maruyama, Noriho Kamiya
1Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, PRESTO, JST, Fukuoka 812-8581, Japan.
Journal of the American Chemical Society
|July 15, 2004
概括
一种使用酶-表面活性剂复合物的新型支持液膜 (SLM) 系统实现了 (S) -ibuprofen和L-phenylalanine从racemic混合物中高度酶选择性分离,其中超过91%的酶过量.
科学领域:
- 生物催化剂是一种生物催化剂.
- 膜科学 膜科学 膜科学
- 螺旋式分离 螺旋式分离
背景情况:
- 对于制药品和精细化学品来说,对酶选择性分离至关重要.
- 传统的性分辨率方法可能是低效或昂贵的.
- 酶催化提供了一种绿色和选择性的方法,用于奇拉分离.
研究的目的:
- 为光学活性化合物开发一种高度分离的分离系统.
- 使用带有封装酶-表面活性剂复合物的支液体膜 (SLM).
- 为了实现高效的光学分辨率的racemic ibuprofen和phenylalanine.
主要方法:
- 支持液体膜 (SLM) 的开发.
- 在SLM中封装表面活性剂-酶复合物 (脂酶或α-chymotrypsin).
- 采用SLM用于从racemic混合物中提取 chiral 化合物的 enantioselective 运输.
主要成果:
- 这种新型SLM系统显示出高的enantioselectivity.
- 对于 (S) -ibuprofen和L-phenylalanine,均达到了超过91%的等离子过量 (ee).
- 膜阶段内的酶有效地驱动了对抗选择性传输.
结论:
- 开发的SLM系统提供了一种有效的合性分辨率方法.
- 在SLM中酶固定是对酶选择性分离的有希望的策略.
- 这种方法提供了一种高度选择性和高效的途径,用于获得光学纯净的化合物.
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