在微型板中对寡糖类数组的共价显示
Marian C Bryan1, Fabio Fazio, Hing-Ken Lee
1Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Journal of the American Chemical Society
|July 15, 2004
概括
一个新的共价阵列使得碳水化合物显示用于研究蛋白质相互作用,包括HIV抗体2G12. 这种方法允许对多价值碳水化合物与蛋白质结合的质谱分析.
科学领域:
- 碳水化合物的化学成分
- 结构生物学是结构生物学.
- 免疫学 免疫学 免疫学
背景情况:
- 复杂的碳水化合物在生物过程中起着至关重要的作用.
- 研究碳水化合物-蛋白质相互作用对于了解艾滋病毒等疾病至关重要.
- 现有的显示碳水化合物的方法可能是有限的.
研究的目的:
- 开发一种多功能共价阵列,用于显示复杂的寡糖.
- 为了研究碳水化合物与蛋白质的相互作用,包括抗体和乳素.
- 描述2G12抗体与其HIV表位的多价值结合.
主要方法:
- 开发用于微型板的共价阵列系统.
- 复杂的寡糖化合物的固定在数组表面上.
- 使用莱克和抗体,包括2G12抗体的结合性测试.
- 用于质谱分析的结合复合物的裂变.
主要成果:
- 成功创建了一个用于寡糖体显示的功能共价阵列.
- 该系统有效地捕获了碳水化合物和蛋白质之间的相互作用.
- 量化了2G12抗体与其最佳表位体的多价值相互作用 (Kd 0.1 μM).
- 质谱法使得分裂复合物的进一步表征成为可能.
结论:
- 共价阵列是研究碳水化合物-蛋白质相互作用的强大工具.
- 这种方法有助于对2G12等针对病毒点的抗体进行调查.
- 该系统允许详细分析多价值结合事件和随后的表征.
相关概念视频
Sugars as Energy Storage Molecules
Sugar (a simple carbohydrate) metabolism (chemical reactions) is a classic example of the many cellular processes that use and produce energy. Living things consume sugar as a major energy source because sugar molecules have considerable energy stored within their bonds. Consumed carbohydrates have their origins in photosynthesizing organisms like plants. During photosynthesis, plants use the energy of sunlight to convert carbon dioxide gas into sugar molecules, like glucose. Because this...
Glucose Transporters
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Glucose Absorption Into the Small Intestine
Complex carbohydrates consumed cannot be absorbed into the small intestine in their original form. First, they must be hydrolyzed to a monosaccharide form such as glucose or galactose. These monosaccharides are then transported across the intestinal membrane and into the blood via transcellular transport. The intestinal epithelial cells allow the movement of these monosaccharides with a defined 'entry' through membrane transporter proteins present on their apical membrane and 'exit' via the...
Histogram
The histogram is a graphical representation in the x-y form of data distribution in a data set. The horizontal x-axis is labeled with what the data represents (for instance, distance from your home to school). The vertical y-axis is labeled either frequency or relative frequency (or percent frequency or probability).
A histogram graph consists of contiguous (adjoining) boxes. The heights of the bars correspond to frequency values. The graph will have the same shape with respective labels. The...
A histogram graph consists of contiguous (adjoining) boxes. The heights of the bars correspond to frequency values. The graph will have the same shape with respective labels. The...
Sugars as Energy Storage Molecules
Sugar (a simple carbohydrate) metabolism (chemical reactions) is a classic example of the many cellular processes that use and produce energy. Living things consume sugar as a major energy source because sugar molecules have considerable energy stored within their bonds. Consumed carbohydrates have their origins in photosynthesizing organisms like plants. During photosynthesis, plants use the energy of sunlight to convert carbon dioxide gas into sugar molecules, like glucose. Because this...
Average Value of a Function
The average value of a function over a closed interval can be interpreted geometrically as the height of a rectangle whose area equals the net area under the curve across that interval. This net area accounts for both positive and negative contributions of the function, providing a single representative value that reflects the function’s overall behaviorA practical illustration of this idea arises when monitoring the temperature inside a greenhouse over a twenty-four-hour period. Although the...


