平面细胞极性信号控制斑马鱼胃流动期间的细胞分裂方向
Ying Gong1, Chunhui Mo, Scott E Fraser
1Biological Imaging Center, Beckman Institute, California Institute of Technology, Pasadena, California 91125, USA.
Nature
|July 16, 2004
概括
斑马鱼的细胞在胃化过程中沿着动物-植物轴分裂,由非正规的Wnt信号驱动. 这种定向的细胞分裂对于脊椎动物的轴延长至关重要.
科学领域:
- 发育生物学是发展生物学.
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 定向细胞分裂对于组织发育和成形至关重要.
- 调节细胞分裂方向的机制尚不清楚,尤其是脊椎动物.
- 之前的研究主要集中在无脊椎动物上,如Caenorhabditis elegans和Drosophila melanogaster.
研究的目的:
- 为了研究调节斑马鱼胃流动期间的线粒分裂方向的机制.
- 确定Wnt信号在确定细胞分裂极性中的作用.
- 探索定向细胞分裂对脊椎动物轴延长的贡献.
主要方法:
- 在消化过程中对斑马鱼胚胎的体内共聚焦成像.
- 在背部组织中分析线粒分裂.
- 研究 Wnt 途径的组成部分 (Silberblick/Wnt11,不整形,).
主要成果:
- 斑马鱼背部组织中的细胞优先沿着动物-植物轴分裂.
- 包括Silberblick/Wnt11,Dishevelled和Strabismus在内的Wnt路径对于建立这种极性至关重要.
- 非正规的Wnt信号传递在斑马鱼胃化过程中在定向细胞分裂中起着重要作用.
结论:
- 定向细胞分裂是斑马鱼轴延长的一个关键驱动因素.
- 非正规的Wnt信号对于调节细胞分裂方向至关重要.
- 非正规的Wnt信号可能在脊椎动物的轴延长中起着保留的作用,影响细胞间隙和分裂.
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