心脏记忆随着年龄的增长而演变,与短暂的外向电流的发展有关
Alexei N Plotnikov1, Eugene A Sosunov, Kornelis W Patberg
1Center for Molecular Therapeutics, Department of Pharmacology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA.
Circulation
|July 21, 2004
概括
心脏记忆 (CM) 随着年龄的增长而发展,这是由于对不敏感的短暂外流 (I(to)) 的成熟. 这一过程涉及到表心动作潜力和相关基因表达的变化,影响心脏的电力重塑能力.
科学领域:
- 心脏病学 心脏病学
- 电子生理学 电子生理学
- 发育生物学 发展生物学
背景情况:
- 对不敏感的短暂向外电流 (I(to)) 在心脏记忆 (CM) 中起着至关重要的作用.
- 心脏记忆反映了心脏的电生理学重塑能力.
- 这项研究研究了I ((to) 的发育成熟,作为CM进化的驱动力.
研究的目的:
- 测试一个假设,心脏记忆的进化是由I的发育成熟驱动的假设.
- 了解心脏中电生理学重塑的年龄相关变化.
主要方法:
- 心脏节奏被用来诱导CM在不同年龄的狗 (1天到成年人).
- 测量T波向量位移 (TVD),以量化CM.
- 微电极研究评估了表心动作潜力的痕特征.
- 使用RT-PCR量化了KChIP2和Kv4.3的mRNA表达.
主要成果:
- 成人TVD显著高于新生儿,并且随着年龄的增长而增加,大约在200天后达到成人水平.
- 在新生儿中缺少的表心动作潜能,变得明显,并且随着年龄的增长而加深.
- 在隙大小和TVD之间观察到正相关性.
- 随着年龄的增长,KChIP2和Kv4.3mRNA水平增加,反映了CM的发展.
结论:
- 心脏记忆的诱导性随着年龄的增长而增加.
- 这种年龄相关的增加与心表动作潜力的突出和KChIP2/Kv4.3mRNA表达的演变有关.
- 心脏在发育过程中对电生理学重塑和记忆的能力部分取决于I(to) 决定因素的成熟.
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