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Updated: Jul 20, 2026

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
07:42

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 19, 2010

通过使用液晶来描绘固定在不同方向的表面上的蛋白质的结合能力

Yan-Yeung Luk1, Matthew L Tingey, Kimberly A Dickson

  • 1Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 53706, USA.

Journal of the American Chemical Society
|July 22, 2004
PubMed
概括

蛋白质在表面上的定位显著影响结合能力. 与随机定向相比,将核糖核酶A (RNase A) 固定在一个首选的方向上,导致核糖核酶抑制蛋白 (RI) 的结合率提高了4倍.

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相关概念视频

Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.