通过环林B-Cdk的酸化是从核中释放线粒退出激活剂Cdc14的基础
Ramzi Azzam1, Susan L Chen, Wenying Shou
1Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
Science (New York, N.Y.)
|July 27, 2004
概括
发芽酵母蛋白酸酶Cdc14是由FEAR网络从核中释放出来的. 这个网络通过循环素依赖激酶 (Cdk) 促进Net1酸化,使得能够及时从线粒分裂中退出.
科学领域:
- 细胞周期调节细胞周期调节
- 米托斯出口的出口.
- 发芽的酵母遗传学 发芽的酵母遗传学
背景情况:
- 蛋白酸酶Cdc14是发芽酵母中线粒性退出的关键.
- 在介相期间,Cdc14被Net1隔离在核中.
- 在Cdc14早期无相释放 (FEAR) 网络控制Cdc14的分散.
研究的目的:
- 调查FEAR网络的监管机制.
- 为了阐明循环林依赖激酶 (Cdk) 在Cdc14释放中的作用.
- 了解Net1酸化如何影响线粒细胞的进展.
主要方法:
- 酵母遗传学和分子生物学技术.
- 蛋白质酸化事件的分析.
- 对细胞循环进展和线粒体事件的观察.
主要成果:
- 费尔网络通过CDK-环素B1/B2复合体促进Net1酸化.
- 网1酸化对于FEAR介导的Cdc14释放至关重要.
- 这些事件对于晚期线粒体事件的及时执行至关重要.
结论:
- 涉及Cdk的调节电路确保了适当的线粒体退出.
- 通过FEAR网络对Net1进行Cdk介导的酸化是关键的控制点.
- 这种机制确保了细胞循环的准确进展.
相关概念视频
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...